Blocking Assay:Article Title: Oxytocin and vasopressin within the ventral and dorsal lateral septum modulate aggression in female rats
Article Snippet: .. Experiment Tissue Blocking solution Primary antibody Secondary antibody Mounting medium Neuronal activity VenusVGAT 5% NGS rabbit-anti-pERK antibody (1:250 CellSignalling #9101 or #4370, Danvers, MA, USA) Alexa-fluor 594 goat- anti-rabbit antibody (1:200, ThermoFisher Scientific, Germany) Vectastain Hard-Set (H- 1400, Vector Laboratories, Inc., Burlingame, CA, USA Patch-clamp Venus- VGAT 5% NGS Streptavidin conjugated with CF633 (1:400; Biotium, Fremont, CA, USA) DAPI Fluoromount-G (SouthernBiotec h, Birmingham, AL, USA) Molecular identification of LS neurons VenusVGAT 5% NGS rabbit-anti-ERα (1:500, C1355, Millipore, USA); chicken-anti- Somatostatin (1:500, 366-006, Synaptic Systems, Gottingen, GER) Alexa-fluor 594 goat- anti-rabbit antibody (1:250; ThermoFisher Scientific, Germany): Alexa-fluor 647 goat- anti-chicken (1:250, ThermoFisher Scientific, Germany) Vectastain Hard-Set (H- 1400, Vector Laboratories, Inc., Burlingame, CA, USA Chemo- and optogenetics Wistar 1% BSA 5% NGS mouse-anti- neurophysin1/oxytocin (1:500, Harold Gainer, p38); rabbit-anti- mCherry (1:1.000, ab167453, Abcam) Alexa-fluor 488 goat- anti-mouse antibody (1:1000, ThermoFisher Scientific, Germany): Alexa-fluor 594 goat- anti-rabbit (1:1000, ThermoFisher Scientific, Germany) DAPI Fluoromount-G (SouthernBiotec h, Birmingham, AL, USA) Supplementary Table 2: Immunohistochemistry specifics: NGS (normal-goat serum), BSA (bovine serum albumin). ..
Activity Assay:Article Title: Oxytocin and vasopressin within the ventral and dorsal lateral septum modulate aggression in female rats
Article Snippet: .. Experiment Tissue Blocking solution Primary antibody Secondary antibody Mounting medium Neuronal activity VenusVGAT 5% NGS rabbit-anti-pERK antibody (1:250 CellSignalling #9101 or #4370, Danvers, MA, USA) Alexa-fluor 594 goat- anti-rabbit antibody (1:200, ThermoFisher Scientific, Germany) Vectastain Hard-Set (H- 1400, Vector Laboratories, Inc., Burlingame, CA, USA Patch-clamp Venus- VGAT 5% NGS Streptavidin conjugated with CF633 (1:400; Biotium, Fremont, CA, USA) DAPI Fluoromount-G (SouthernBiotec h, Birmingham, AL, USA) Molecular identification of LS neurons VenusVGAT 5% NGS rabbit-anti-ERα (1:500, C1355, Millipore, USA); chicken-anti- Somatostatin (1:500, 366-006, Synaptic Systems, Gottingen, GER) Alexa-fluor 594 goat- anti-rabbit antibody (1:250; ThermoFisher Scientific, Germany): Alexa-fluor 647 goat- anti-chicken (1:250, ThermoFisher Scientific, Germany) Vectastain Hard-Set (H- 1400, Vector Laboratories, Inc., Burlingame, CA, USA Chemo- and optogenetics Wistar 1% BSA 5% NGS mouse-anti- neurophysin1/oxytocin (1:500, Harold Gainer, p38); rabbit-anti- mCherry (1:1.000, ab167453, Abcam) Alexa-fluor 488 goat- anti-mouse antibody (1:1000, ThermoFisher Scientific, Germany): Alexa-fluor 594 goat- anti-rabbit (1:1000, ThermoFisher Scientific, Germany) DAPI Fluoromount-G (SouthernBiotec h, Birmingham, AL, USA) Supplementary Table 2: Immunohistochemistry specifics: NGS (normal-goat serum), BSA (bovine serum albumin). ..
Next-Generation Sequencing:Article Title: Oxytocin and vasopressin within the ventral and dorsal lateral septum modulate aggression in female rats
Article Snippet: .. Experiment Tissue Blocking solution Primary antibody Secondary antibody Mounting medium Neuronal activity VenusVGAT 5% NGS rabbit-anti-pERK antibody (1:250 CellSignalling #9101 or #4370, Danvers, MA, USA) Alexa-fluor 594 goat- anti-rabbit antibody (1:200, ThermoFisher Scientific, Germany) Vectastain Hard-Set (H- 1400, Vector Laboratories, Inc., Burlingame, CA, USA Patch-clamp Venus- VGAT 5% NGS Streptavidin conjugated with CF633 (1:400; Biotium, Fremont, CA, USA) DAPI Fluoromount-G (SouthernBiotec h, Birmingham, AL, USA) Molecular identification of LS neurons VenusVGAT 5% NGS rabbit-anti-ERα (1:500, C1355, Millipore, USA); chicken-anti- Somatostatin (1:500, 366-006, Synaptic Systems, Gottingen, GER) Alexa-fluor 594 goat- anti-rabbit antibody (1:250; ThermoFisher Scientific, Germany): Alexa-fluor 647 goat- anti-chicken (1:250, ThermoFisher Scientific, Germany) Vectastain Hard-Set (H- 1400, Vector Laboratories, Inc., Burlingame, CA, USA Chemo- and optogenetics Wistar 1% BSA 5% NGS mouse-anti- neurophysin1/oxytocin (1:500, Harold Gainer, p38); rabbit-anti- mCherry (1:1.000, ab167453, Abcam) Alexa-fluor 488 goat- anti-mouse antibody (1:1000, ThermoFisher Scientific, Germany): Alexa-fluor 594 goat- anti-rabbit (1:1000, ThermoFisher Scientific, Germany) DAPI Fluoromount-G (SouthernBiotec h, Birmingham, AL, USA) Supplementary Table 2: Immunohistochemistry specifics: NGS (normal-goat serum), BSA (bovine serum albumin). ..
Patch Clamp:Article Title: Oxytocin and vasopressin within the ventral and dorsal lateral septum modulate aggression in female rats
Article Snippet: .. Experiment Tissue Blocking solution Primary antibody Secondary antibody Mounting medium Neuronal activity VenusVGAT 5% NGS rabbit-anti-pERK antibody (1:250 CellSignalling #9101 or #4370, Danvers, MA, USA) Alexa-fluor 594 goat- anti-rabbit antibody (1:200, ThermoFisher Scientific, Germany) Vectastain Hard-Set (H- 1400, Vector Laboratories, Inc., Burlingame, CA, USA Patch-clamp Venus- VGAT 5% NGS Streptavidin conjugated with CF633 (1:400; Biotium, Fremont, CA, USA) DAPI Fluoromount-G (SouthernBiotec h, Birmingham, AL, USA) Molecular identification of LS neurons VenusVGAT 5% NGS rabbit-anti-ERα (1:500, C1355, Millipore, USA); chicken-anti- Somatostatin (1:500, 366-006, Synaptic Systems, Gottingen, GER) Alexa-fluor 594 goat- anti-rabbit antibody (1:250; ThermoFisher Scientific, Germany): Alexa-fluor 647 goat- anti-chicken (1:250, ThermoFisher Scientific, Germany) Vectastain Hard-Set (H- 1400, Vector Laboratories, Inc., Burlingame, CA, USA Chemo- and optogenetics Wistar 1% BSA 5% NGS mouse-anti- neurophysin1/oxytocin (1:500, Harold Gainer, p38); rabbit-anti- mCherry (1:1.000, ab167453, Abcam) Alexa-fluor 488 goat- anti-mouse antibody (1:1000, ThermoFisher Scientific, Germany): Alexa-fluor 594 goat- anti-rabbit (1:1000, ThermoFisher Scientific, Germany) DAPI Fluoromount-G (SouthernBiotec h, Birmingham, AL, USA) Supplementary Table 2: Immunohistochemistry specifics: NGS (normal-goat serum), BSA (bovine serum albumin). ..
Optogenetics:Article Title: Oxytocin and vasopressin within the ventral and dorsal lateral septum modulate aggression in female rats
Article Snippet: .. Experiment Tissue Blocking solution Primary antibody Secondary antibody Mounting medium Neuronal activity VenusVGAT 5% NGS rabbit-anti-pERK antibody (1:250 CellSignalling #9101 or #4370, Danvers, MA, USA) Alexa-fluor 594 goat- anti-rabbit antibody (1:200, ThermoFisher Scientific, Germany) Vectastain Hard-Set (H- 1400, Vector Laboratories, Inc., Burlingame, CA, USA Patch-clamp Venus- VGAT 5% NGS Streptavidin conjugated with CF633 (1:400; Biotium, Fremont, CA, USA) DAPI Fluoromount-G (SouthernBiotec h, Birmingham, AL, USA) Molecular identification of LS neurons VenusVGAT 5% NGS rabbit-anti-ERα (1:500, C1355, Millipore, USA); chicken-anti- Somatostatin (1:500, 366-006, Synaptic Systems, Gottingen, GER) Alexa-fluor 594 goat- anti-rabbit antibody (1:250; ThermoFisher Scientific, Germany): Alexa-fluor 647 goat- anti-chicken (1:250, ThermoFisher Scientific, Germany) Vectastain Hard-Set (H- 1400, Vector Laboratories, Inc., Burlingame, CA, USA Chemo- and optogenetics Wistar 1% BSA 5% NGS mouse-anti- neurophysin1/oxytocin (1:500, Harold Gainer, p38); rabbit-anti- mCherry (1:1.000, ab167453, Abcam) Alexa-fluor 488 goat- anti-mouse antibody (1:1000, ThermoFisher Scientific, Germany): Alexa-fluor 594 goat- anti-rabbit (1:1000, ThermoFisher Scientific, Germany) DAPI Fluoromount-G (SouthernBiotec h, Birmingham, AL, USA) Supplementary Table 2: Immunohistochemistry specifics: NGS (normal-goat serum), BSA (bovine serum albumin). ..
Immunohistochemistry:Article Title: Oxytocin and vasopressin within the ventral and dorsal lateral septum modulate aggression in female rats
Article Snippet: .. Experiment Tissue Blocking solution Primary antibody Secondary antibody Mounting medium Neuronal activity VenusVGAT 5% NGS rabbit-anti-pERK antibody (1:250 CellSignalling #9101 or #4370, Danvers, MA, USA) Alexa-fluor 594 goat- anti-rabbit antibody (1:200, ThermoFisher Scientific, Germany) Vectastain Hard-Set (H- 1400, Vector Laboratories, Inc., Burlingame, CA, USA Patch-clamp Venus- VGAT 5% NGS Streptavidin conjugated with CF633 (1:400; Biotium, Fremont, CA, USA) DAPI Fluoromount-G (SouthernBiotec h, Birmingham, AL, USA) Molecular identification of LS neurons VenusVGAT 5% NGS rabbit-anti-ERα (1:500, C1355, Millipore, USA); chicken-anti- Somatostatin (1:500, 366-006, Synaptic Systems, Gottingen, GER) Alexa-fluor 594 goat- anti-rabbit antibody (1:250; ThermoFisher Scientific, Germany): Alexa-fluor 647 goat- anti-chicken (1:250, ThermoFisher Scientific, Germany) Vectastain Hard-Set (H- 1400, Vector Laboratories, Inc., Burlingame, CA, USA Chemo- and optogenetics Wistar 1% BSA 5% NGS mouse-anti- neurophysin1/oxytocin (1:500, Harold Gainer, p38); rabbit-anti- mCherry (1:1.000, ab167453, Abcam) Alexa-fluor 488 goat- anti-mouse antibody (1:1000, ThermoFisher Scientific, Germany): Alexa-fluor 594 goat- anti-rabbit (1:1000, ThermoFisher Scientific, Germany) DAPI Fluoromount-G (SouthernBiotec h, Birmingham, AL, USA) Supplementary Table 2: Immunohistochemistry specifics: NGS (normal-goat serum), BSA (bovine serum albumin). ..
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